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Fig. 1. Cloning of mog-6. (A) Genetic map. mog-6 is located on chromosome II, between unc-4 and sqt-1. (B) Deletion analysis. mnDf66, mnDf89 and mnDf90 (black) remove mog-6 while mnDf29, mnDf57, mnDf62 and mnDf87 (gray) do not. The exact breakpoints of mnDf62 and mnDf66 have not been determined (dashed lines). (C) Cosmids tested for rescue (black bars); only M106 rescued mog-6. (D) Subcloning of M106. A 3.1 kb fragment (pAP6) of M106 rescued mog-6 and contains one open reading frame. (E) The mog-6 gene. 5' and 3' flanking regions are represented as lines, exons as gray boxes, introns as lines joining the exons.





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