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Fig. 2. (A) The MOG-6 protein. The CBD is highlighted in gray (AA275-434). Residues that are involved in Cyclosporin A (CsA)-binding in canonical cyclophilins are shown in bold. The nuclear localization signal is overlined. The starting point of the deletion in mog-6(q465) is shown by an arrow. The sequences of MOG-6 and of its human ortholog hCyp-60 are aligned. Conserved residues are boxed. (B) Western analysis. A 64 kD protein was detected with affinity-purified anti-MOG-6 antibodies in wild-type adults and masculinized adults (fem-3(q96gf)), but was absent in mog-6(q465) mutant adults. A minor 42 kD protein (open arrow) was absent in q465 extracts but was also detected with preimmune serum (not shown). White arrowheads indicate cross-reacting bands that are oogenesis-dependent, since absent in masculinized animals.





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