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Fig. 5. Analysis of RNA splicing in mog-6 mutants. (A) RT-PCR. Genomic DNA
(D) or polyA-enriched RNA (A+) from wild-type worms were used as
positive controls for PCR with sets of oligonucleotides specific for either
fem-3 (lanes 1 to 6), fbf-2 (lanes 7 to 12), nos-3
(lanes 13 to 18) or ceh-13 (lanes 19 to 24). Total RNA from either
mog-6 or wild-type adults (wt) was used for PCR without (–) or
with (+) RT. PCR on genomic DNA was used as a reference for the size of
unspliced RNAs (lanes 1, 7, 13 and 19). (B) Northern analysis. Total RNA, 12.5
µg, derived from adult mog-6 or wild-type worms were run on a
denaturing agarose gel and probed for mog-6, fbf, nos-3, ges-1 and
actin. (C) gld-3 transcripts were analyzed on a separate blot loaded
with 25 µg and 50 µg of total RNA from wild-type and mog-6
mutants, respectively. The two major gld-3 transcripts are indicated
(Small and Large). Blots were also overexposed to ensure that no minor
transcripts of abnormal size were present (not shown).
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