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Fig. 6. Selected examples of the localization of GFP-tagged Dsh mutants. Deletion of the C-terminus does not affect VCL (A, arrow) and deletion of the PDZ domain only partially abrogates targeting (B, arrow). VCL is completely blocked by deletion of the DIX domain (C) or the region between the PDZ and DEP domains (D). (E) VCL is also blocked by introducing two point mutations (K57A/E58A) into the phospholipid-binding motif within the DIX domain. (F) Co-injection of LvDsh.WT.GFP and the DIX domain of LvDsh shows that the latter does not exert its dominant negative effect by blocking VCL.





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