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Fig. 7. Dsh function is required for endomesoderm specification and for the
accumulation of ß-catenin in the nuclei of vegetal blastomeres. (A)
Injection of mRNA (1.9 mg/ml) encoding the DIX domain of LvDsh resulted in
suppression of endoderm and mesoderm formation and a phenotype
indistinguishable from that produced by overexpression of cadherins or
GSK3ß (Emily-Fenouil et al.,
1998; Wikramanayake et al.,
1998; Logan et al.,
1999). (B,C) Overexpression of the PDZ domain of LvDsh (2.0 mg/ml
mRNA) or the DIX domain of LvAxin (3.6 mg/ml mRNA) did not produce apparent
phenotypes. Embryos shown in (A-C) are at 20 hours, 20 hours and 18 hours of
development, respectively. (D,E) Overexpression of the DIX domain of LvDsh
blocked the nuclear accumulation of ß-catenin in vegetal blastomeres
(arrow, E), as shown by immunostaining using an anti-Lv-ß-catenin
antibody.
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