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Fig. 2. (A-C) Flat preparations of her3::gal4 embryos (tail-bud, A;
two-somite stage, B; six-somite stage, C) labelled by in situ hybridisation
with a gal4 probe. The gal4 pattern closely corresponds to
the endogenous her3 transcription pattern. Compare with
Fig. 1. The arrows in C
indicate areas of gal4 expression in epidermal tissues. In cranial
regions of the developing spinal cord, reporter mRNA persists for longer than
do the transcripts of the endogenous her3 gene (see
Fig. 1). (D,E) Two examples of
her3::gal4 embryos injected with mRNA for notch1a-intra and probed
for gal4. Note that transcription of her3::gal4 is
repressed. Anterior is towards the top.
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