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Fig. 9. KS117 GFP marker expression 9 days after pollination of heterozygous gametophytic maternal mutants by a demethylated genome. (A) mea-6/MEA x MET1 a/s; (B) fis2-6/FIS2 x MET1 a/s; (C) fie-11/FIE x MET1 a/s; (D) dme-4/DME x MET1 a/s; (E) bga-1/BGA x MET1 a/s; (F) msi1-2/MSI1 x MET1 a/s. Two types of seeds are produced when fis/FIS mutants are fertilised by demethylated MET1 a/s pollen: the small seeds (arrows) result from WT ovule development, the large seeds (arrowheads) from mutant ovules. GFP expression is low and restricted to the posterior pole in small seeds. In mea/MEA (A), fis2/FIS2 (B), dme/DME (D) and bga/BGA (E) seeds, KS117 GFP expression is properly restricted to the cyst, even when this structure is larger than in WT (A). In contrast, in fie/FIE (C) and msi1/MSI1 (F) seeds, KS117 GFP is over-expressed and not restricted to the posterior cyst (arrowheads), as when pollinated by WT. Scale bar: 200 µm (all images).

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