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Fig. 1. KANADI loss-of-function morphological phenotypes. (A-C) All three KANADI
genes, KAN1 (A), KAN2 (B), and KAN3 (C) are
expressed in the abaxial regions of developing embryos. Only one presumptive
cotyledon is visible in the section showing KAN3 expression. Unlike
the mild or lack of phenotypic alterations of the single mutants kan1
and kan2, respectively, kan1-2 kan2-1 plants exhibit gross
morphological aberrations in all lateral organs. (D) A kan1-2 kan2-1
plant with narrow leaves which have outgrowths formed on their abaxial side
(D). (G,H) The abaxial outgrowths (arrows) are visible shortly after leaf
primordia have expanded, appearing first as a row along the bottom third of
the leaf, and later in a less organized pattern as the leaf elongates. (E) A
wild-type plant. (F) In wild-type leaves, two stipules (*) are formed on the
flanks of each leaf, obscuring the apical meristem from view. (I) Leaves of
kan1-2 kan2-1 kan3-1 plants exhibit much less lamina expansion than
do those of the double mutant, being radial except at their distal tips. (L)
Leaves of the triple mutant are radial at inception, but they still retain
some polar characteristics, such as a lack of abaxial trichomes on the first
two leaves. (J-L) As in phb-1d homozygotes, axillary meristems
(arrows) may form on the abaxial sides of kan1 kan2 kan3 leaves (J,K)
and similar to kan1-2 kan2-1 and phb-1d/+ leaves, stipules
(arrows) develop all around the base of the leaves (L). (M) Disorganized blade
tissue often forms at the distal tips of kan1 kan2 kan3 leaves. ab,
abaxial; ad, adaxial.
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