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Fig. 2. KANADI loss-of-function anatomical phenotypes. (A,B) Longitudinal and
transverse sections of 12-day-old wild-type seedlings demonstrate that young
leaf primordia exhibit polarity along the ab/ad axis immediately after their
separation from the shoot apical meristem. Polarity is evident both by their
crescent shape and the appearance of vacuolated cells (arrows) on the abaxial
side first (B). (C) In differentiating leaves, asymmetric anatomy along the
ab/ad axis is evident in the shape of the adaxial palisade mesophyll versus
the abaxial spongy mesophyll. Anticlinal cell divisions characterize the L1
and L2 cell layers both adaxially and abaxially (insert; arrow). (D,E) In
contrast, leaf primordia of kan1-2 kan2-1 12-day-old seedlings appear
radial (arrows), with all cells maintaining their densely cytoplasmic
appearance for a prolonged period. (F) Many more cell layers are found in
differentiating leaves, resulting from abnormal periclinal divisions at the
abaxial side (insert; arrows). (G-I) Leaf primordia of kan1-2 kan2-1
kan3-1 seedlings (G,H) are also radial at inception (arrows), but do
exhibit some asymmetric growth later in development such that the leaves are
also thicker than those of wild type (I). The transverse sections of the
leaves in C, F, and I are from the proximal region of expanding leaves of
12-day-old seedlings. *, stipules; ab, abaxial; ad, adaxial; lp, leaf
primordia; m, meristem; pm, palisade mesophyll; sm, spongy mesophyll. Scale
bars: 50 µm.
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