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Fig. 4. The nature of the kan1 kan2 blade outgrowths. (A) The outgrowths
developing on the abaxial side of kan1-2 kan2-1 leaves appear nearly
radial, with cell types normally found on leaf margins positioned around their
entire circumference. (B) In wild-type seedlings, these cells show blue
staining when the GUS reporter is driven by the enhancer trap YJ158. (C) This
reporter drives GUS throughout the epidermis of the kan1 kan2 blade
outgrowths and in scattered cells on the abaxial leaf surface. (D) Transverse
section through a mature wild-type leaf displaying an asymmetric anatomy both
within the leaf and in the vascular bundle. (E,F) In mature kan1 kan2
leaves, numerous vascular bundles are formed, connecting the outgrowths to
leaf main bundles (E). The radial outgrowths have nearly radial anatomy, with
a large bundle (arrow) found in their center (F). (G) Close-up of a wild-type
minor leaf bundle showing xylem vessel members (arrow) positioned adaxially,
while phloem sieve tube elements (arrow) are located abaxially. (H) In
kan1 kan2 the central bundles of the outgrowths have clusters of
phloem tissue surrounding xylem vessel members. (I,J) The prolonged period of
cell division in the abaxial region of kan1 kan2 leaves is
responsible for the formation of outgrowths, reflected by the maintenance of
the densely cytoplasmic appearance of these cells (I), and correlated with
prolonged localized FIL mRNA expression (J). (K) Earlier in leaf
development, high levels of FIL expression demarcate presumptive
outgrowths (arrow) while a lower level of FIL expression is
throughout the abaxial region of the leaves. ab, abaxial; ad, adaxial; ph,
phloem; xy, xylem. Scale bars: 1 mm (A), 50 µm (B-K).
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