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Fig. 5. Parallels between Drosophila dorsal closure and wound healing. (A)
Confocal micrograph of a dorsal closure stage Drosophila embryo
expressing GFPactin to reveal the actin cable and filopodial protrusions that
drive dorsal closure. (B) A transmission electron micrograph section cut
through the zippering zone shows how the filopodia of opposing epithelial
cells (arrows) interdigitate and prime the formation of adhesions between the
two epithelial fronts. (C,D) Equivalent images from laser wounds in similarly
staged embryos that show how opposing epithelial fronts (arrows in D) are
knitted together using the same actin-based machineries as for dorsal closure.
(E) A temporal series that illustrates how filopodial interdigitation is
believed to prime the assembly of mature adherens junctions. Adjacent cells
extend filopodia towards each other, which interdigitate, with actin (red),
catenins and cadherins (yellow) localizing to the filopodial tips and points
of contact. The filopodia then shorten, drawing the cells together. This
filopodial zippering is propagated to the edge of the cell resolving into
mature junctions. (A-D) Courtesy of Will Wood; (E) courtesy of Craig
Magie.
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