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Fig. 2. XStau is expressed in Xenopus oocytes. (A) Total oocyte S10 lysate was immunoblotted with either preimmune sera (lane 1) or XStau antibodies (lane 2). A single band of ~80 kDa is recognized by XStau antibodies (lane 2). Molecular weight markers are indicated at the left. (B) Oocyte S10 lysates were prepared from each stage of oogenesis [I-VI (Dumont, 1972)]. One oocyte equivalent of S10 lysate per lane was resolved by SDS-PAGE and immunoblotted using XStau antibodies (above) or tubulin antibodies (below).





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