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Fig. 3. Oscillations in FAD++ autofluorescence are stimulated by
fertilisation induced Ca2+ transients. (A) Variations in
[Ca2+]c (measured with indo 1 AM, blue trace) and
FAD++ autofluorescence (green trace) observed at fertilisation of a
mouse egg (n=20). Time 0 corresponds to the time of insemination. (B)
Variations in [Ca2+]i (measured with rhod 2 AM, red
trace) and FAD++ autofluorescence (green trace) in a mature mouse
egg injected with caged Ins(1,4,5)P3 (n=10). A UV
flash (red arrowhead) releases Ins(1,4,5)P3 in the egg and
triggers a Ca2+ transient accompanied by a transient decrease in
FAD++ autofluorescence. i and ii show the same egg as in
Fig. 2D-F with the
FAD++ signal (i) and Rhod2 AM (ii) signal that have markedly
different distributions. This suggests that, under our conditions, rhod2 AM
does not partition into mitochondria of the mouse egg. The ROI (white circle
in i) used to obtain the measurements is drawn. (C) Oscillations of
FAD++ autofluorescence are stopped by the addition of 5 µM BAPTA
AM to the chamber (the presence of BAPTA AM is indicated by a bar under the
graph, n=8)
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