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Fig. 5. Effect of energetic substrates starvation on sperm-triggered [Ca2+] oscillations and cellular [ATP]. (A,B) Mature eggs are incubated in M2 medium without lactate, pyruvate, glucose and amino acids for 2 hours and then fertilised. Where indicated, 2 mM pyruvate is added to the dish. The lack of energetic substrates disrupts the sperm-triggered [Ca2+] oscillations with either [Ca2+]i remaining high after a Ca2+ transient (A, n=12) or with [Ca2+]i remaining low (B, n=11). (C) Changes in [ATP]i upon withdrawal of energetic substrates (n=7). An egg was placed in M2 medium without energetic substrates for 30 minutes before the start of the recording, then, where indicated, 2 mM pyruvate or 1 µM FCCP was added.





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