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Fig. 6. Fertilisation does not cause a decrease in ATP levels in single eggs. (A,B) Measurements of bulk cytosolic [ATP] in fertilised eggs (A, n=9; B, n=16). Luminescence recording of an egg injected with firefly luciferase. Sperm (S) was added when indicated by the arrow and, in B, FCCP was added when indicated by the second arrow. The time of fertilisation is ~15-30 minutes after sperm addition. Fertilisation was confirmed by the presence of the second polar body 90 minutes after sperm addition.





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