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Fig. 5. The transactivation domain of Sox17ß is required for ß-catenin binding. COS-1 cells were transfected with (A) 3 µg of DNA encoding the indicated V5-epitope tagged Sox17ß constructs or (B) 3 µg of DNA encoding the indicated V5-epitope tagged Sox17{alpha} constructs. The resulting cell extracts were either incubated with GST-agarose or GST-ß-catenin-agarose. The input and bound V5-tagged proteins were visualized by anti-V5 immunoblotting. Mutations or deletion of the conserved transactivation motif in either Sox17ß or Sox17{alpha} impairs or abolishes ß-catenin binding.





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