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Fig. 5. Progenitor location prior to gastrulation correlates with location of progeny in LPM. (A-D) Lateral views at tailbud stage, rostral towards the top. Blue precipitate indicates locations of cells derived from labeled blastomeres. (E-H) Higher magnification views of embryos shown in (A-D). Blastomeres located 62-86° (A,E) or 67-95° (B,F) from dorsal in tier 1 contribute to rostral and medial regions of the LPM. By contrast, blastomeres located 94-120° (C,G) or 120-144° (D,H) from dorsal in tier 3 contribute to relatively caudal and lateral regions of the LPM. (I-M) Dorsal views of embryos at 22 somites (I-K,M) and 20 somites stages (L), anterior towards the top. In situ hybridization indicates expression of cmlc2 (I,L,M), vmhc (J),and amhc (K) with a blue (I-K) or pink (L,M) precipitate. Progeny of labeled blastomeres are indicated with a blue precipitate (L,M). cmlc2 is expressed in all cells of the myocardial cone (I), with more robust expression in the central region that also expresses vmhc (J) than in the peripheral portion that also expresses amhc (K) (Yelon et al., 1999; Berdougo et al., 2003). (L) Blastomeres located 101-107° from dorsal in tier 1 contribute to the central region of the myocardial cone. (M) By contrast, blastomeres located 110-116° from dorsal in tier 3 contribute to the peripheral region of the myocardial cone.

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