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Fig. 2. Disorganized cerebral cortex in the Psen1 mutant. (A-D)
Nissl-stained coronal sections through wild-type (A,C) and Psen1
mutant (B,D) embryos at E16.5 (C and D are higher magnifications of A and B).
The ventricular zone, subventricular zone, intermediate zone and cortical
plate appear disorganized in the mutant. (E,F) Psen1 is expressed in
the ventricular zone, the intermediate zone where cells migrate, the subplate
and the cortical plate in E17.5 wild-type embryos. (F) A higher magnification
of the area indicated by a black box in E. Sagittal sections; anterior is
towards the left. (G,H) BrdU incorporation at E17.5 reveals dense compact
bands of BrdU-positive cells in the wild-type (G) and their abnormal patchy
distribution in the mutant (H), where bands are disrupted (arrowheads in G,H
indicate the ventricular zone). Coronal sections. (I-L) Cortical marker
Scip, detected by in situ hybridization on coronal sections of E17.5
embryos, is expressed in the subventricular zone and cortical plate in the
wild-type (I,K) but reveals many neurons at ectopic positions in intermediate
zone of the mutant (J,L). (K,L) Magnified views of I,J. vz, ventricular zone;
svz, subventricular zone; iz, intermediate zone; sp, subplate; cp, cortical
plate; mz, marginal zone.
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