|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 4. Radial glia abnormalities. (A-D) Radial glia are labeled by DiI injection
in the pial surface of E16.5 cortex, sectioned in the coronal plane (100
µm) after diffusion of the dye. In wild type, radial glia fibers are
regular and labeled in their entire length from the pial to the ventricular
surface (A,C), but appear tangled and often fail to label all the way to the
ventricular surface in the Psen1 mutant (B,D). Two examples of
aggregated fibers are shown in D. (E,F) Radial glial morphology is revealed by
RC2 immunofluorescence on horizontal sections through E14.5 cortex. Radial
glial processes are smooth and long in the wild-type (E, arrowheads), but
irregular in the mutant (F). Notice intense staining towards the radial glial
end-feet. Scale bar: 50 µm. (G,H) DiI was photoconverted on sections
equivalent to those shown in (A,B) and Scip-expressing cells were
detected by in situ hybridization. In wild type (G), glial fibers (brown) are
regular. Black arrows indicate Scip-positive neurons (blue), which
are also DiI labeled, presumably because the dye spread into the neurons from
the radial glia to which they are attached. In the mutant (H), radial glial
fibers aggregate and run at angles to one another (black arrow).
Scip-positive cells (blue) are massed within the radial glial
aggregates. White arrow in H indicates several Scip-positive cell
bodies. All sections are shown with pial side upwards.
|
