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Fig. 6. Midbrain development. (A-D) Whole-mount in situ hybridization on E11.5
embryos. Expression of molecular markers in the ventral midbrain in wild type
(A,C) and Psen1 mutant (B,D) embryos. (A,B) F-spondin expression is
specifically downregulated in the ventral midbrain (and diencephalon), but
unaffected in the hindbrain of the mutant. (C,D) By contrast, no differences
are detected in the expression pattern of Lmx1a between wild type (C)
and mutant (D). (E-H) Anlagen of midbrain nuclei are formed abnormally in the
Psen1 mutants. (E,F) Flat-mount preparations of E11.5 embryos,
ventricular view; anterior towards the top. The oculomotor complex, which is
detected by Gata3 expression, is smaller in the mutant (F) in
comparison with wild type (E). (G-J) Midbrain dopaminergic neurons (DA),
detected by Th expression at E11.5, develop close to the ventral
midline in the wild-type (G) but are continuous across the midline in the
mutant (H). (I,J) At late embryonic stages (E17.5), DA neurons remain
clustered at the midline of the mutant (J). Notice ectopic cluster of
Th-positive neurons (arrow in J). Coronal sections through E11.5
(G,H) and E17.5 (I,J) embryos. aq, aqueduct; red arrowheads in H,J indicate
the midline.
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