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Fig. 2. Two types of NS-dividing cells and two types of P_S-div+P_NS-div divisions. (A) Type IIa division gives the parent’s basal process (BP) to a NS-dividing daughter cell. Daughter cell ‘a’ generated in an E13 slice inherited the BP (solid arrow) from the original progenitor cell and divided away from the ventricular surface (designated as ^BPP_NS-div) giving rise to two daughter cells (solid arrowheads). Daughter cell ‘b’ (designated as P_S-div) extended a new radial process (open arrow) to the pial surface and may have divided at the ventricular surface to generate daughter cells in the ventricular zone (VZ) (open arrowheads). (B) A singly DiI-labeled ^BPP_NS-div cell viewed in greater detail in an E13 slice. Bipolar-to-unipolar transition was evident before NS division (confirmed by pH3 expression, B'). (C) Type IIb division gives the parent’s BP to a S-dividing daughter cell. The process-inheriting daughter cell (‘a’) divided at the ventricular surface (^BPP_S-div, daughter cells are indicated by open arrowheads), whereas its sister cell (‘b’) that did not inherit the process (magnified in C' and C') divided abventricularly (P_NS-div, daughter cells are indicated by solid arrowheads). Of the four granddaughter cells, only one of the daughters generated by ‘a’ was labeled with BrdU added into culture from 28.5 hours to 34.5 hours (immunostained at 34.5 hours, not shown). See also Fig. 1E for type IIb cellular behaviors. (D) A P_NS-div cell singly labeled with DiI on the ventricular surface underwent ventricular process collapse (4.1 to 10.0 hours).

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