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Fig. 3. Morphology and origin of NS-divisions in vivo. (A) Double immunostaining of
an E13 cerebral wall section with phosphorylated vimentin (p-vim) and pH3,
showing two pia-connected, unipolar-shaped dividing cells (one of them is
magnified in A'). (B) A serial section (20 µm thick) from an E14
cerebral hemisphere subjected to GFP-adenovirus injection into the lateral
ventricle 19 hours earlier visualized under a fluorescent microscope. Anti-GFP
antibody detected only the indicated mitosis (telophase, strongly positive for
Ki67; B') in three serial sections. (C) Visualization of cerebral wall
sections from embryos infected with GFP-adenovirus demonstrates
GFP+ cells in both S and NS positions. Scale bars: 10 µm in A,C;
100 µm in B.
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