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Fig. 4. Immunohistochemical and behavioral characterization of daughter cells
generated from NS-dividing cells. (A) A BPPNS-div cell
observed in an E13 slice adopted a unipolar shape and subsequently divided to
produce two Hu+ cells, one of which inherited the pia-connected
process. Panels A' and A' show merged views of confocal images
taken using a 40x (A') or 100x (A'') objective lens.
(B) After undergoing bipolar-to-unipolar change, a
BPPNS-div cell captured in an E13 slice generated two
Hu+ cells (B', 100x), one of which (cell
‘a’) ascended more quickly than its sister (cell ‘b’),
having entered the immature cortical plate (CP) by 20.4 hours. (C) A
PNS-div cell observed in an E14 slice. Division may have occurred
by 5.0 hours. Both daughter cells initially had two or three non-radially
oriented processes in the subventricular zone (SVZ) (10.0-21.0 hours) and
later became more radially oriented and Hu+ [C', 40x;
C'', photographed at three different planes (100x)] in the lower
intermediate zone (28.6 hours), resembling locomotion neurons
(Nadarajah et al., 2001).
Scale bars: 10 µm in A',A',B',C',C'.
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