DEV01187 Supplemental Data

Files in this Data Supplement:

• Figure S1 -

  Fig. S1. Map-based cloning of HEN3. (A) Mapping of hen3-1 using molecular markers. The genomic region containing HEN3 is represented by the thick line on the top. A BAC/P1 contig covering this region is shown below. The numbers represent the numbers of recombination breakpoints between hen3-1 and the markers in either 504 or 1004 chromosomes assayed. HEN3 is At5g63610 located on MBK5. p7 and p8 are two primers used to amplify the HEN3 genomic DNA for rescuing the hen3-1 mutant. (B) Dissected, fully elongated siliques from various genotypes, showing that the HEN3g and HEN3-HA transgenes rescue the hen3-1 silique length defects.

• Figure S2 -

  Fig. S2. Nine-day-old seedlings of wild-type (A, C) and hen3-1 (B, D) plants examined by scanning electron microscopy (SAM) (A,B) and in situ hybridization using histone H4 as the probe (C,D). Older leaves were dissected away to expose the SAM in A,B. Scale bars: 10 mm in A,B; 50 mm in C,D).

• Figure S3 -

  Fig. S3. Ten-day-old seedlings of wild type (Ler) and hen3-1 plants. Root growth is similar between the two genotypes.