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Fig. 5. Ds function is required for the early activation of zfh2, a target gene of Wg pathway. (A-D) Third instar wing discs containing ds^D36 clones marked by absence of lacZ expression (red). (A,B) Early-induced ds^D36 clones (36±12 hours AEL) eliminate zfh2 expression in the hinge region. Observe that the ds mutant territory overproliferates with respect to the wild type. Inset shows Zfh2 expression in wild-type wing disc. (C,D) Late-induced ds^D36 clones (60±12 hours AEL) only downregulate zfh2 expression at the proximal hinge region (asterisk in D), whereas in the pleura region its expression looks unaffected (arrowheads in D). (B,D) Green channels show the expression of Zfh2.

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