|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 1. Generation and phenotypic analysis of a hypomorphic Tbx1 mutant.
(A) Gene targeting strategy used to generate the Tbx1neo
allele. (B) Generation of the loxP-flanked (flox) Tbx1flox
allele. (C) RT-PCR shows low amount of Tbx1 transcript expressed by
the Tbx1neo allele. The RNA was extracted from whole E10.5
embryos. (D-F) Alignment of the OFT in E18.5 embryos; in a
Tbx1+/+ embryo (D) the aorta (ao) communicates with the
left ventricle (LV), in a Tbx1neo/neo mutant (E) with
correct alignment, the truncus (T) communicates with both left and right
ventricles (RV). However, in the Tbx1neo/neo embryo shown
in F, the truncus communicates only with the RV (as in
Tbx1–/– embryos, not shown). (G-I)
Intracardiac ink injection of E11.5 embryos shows normal OFT alignment in a
Tbx1+/+ embryo (G), lack of alignment in a
Tbx1–/– embryo (I), and an intermediate
phenotype in a Tbx1neo/neo embryo (H). av:
atrioventricular valves; pt: pulmonary trunk; sv: semilunar valves; VSD:
ventricular septum defect. Scale bars: 0.5 mm.
|
