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Fig. 5. Myocyte differentiation in OFT of conditional mutants. (A-C) Immunohistochemistry with an anti-{alpha}-smooth muscle actin antibody on coronal sections of E12.5 control (Tbx1flox/–) (A-C) and conditional mutant (A'-C') embryos at the level of the conus (A,A'), conal-truncal transition (B,B') and truncus (C,C'). Note the reduced immunoreactivity in conditional mutants (A'-C'), especially at the conal-truncal transition. Scale bars: 100 µm.





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