|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 1. Lack of melanocyte differentiation in
EdnrblacZ/EdnrblacZ neural crest cells
in culture. Primary cultures from embryos of the indicated genotypes were
established and kept in the presence of EDN3 for 2 weeks before fixation.
X-gal staining was then performed and the cultures were photographed in bright
field to show melanin and ß-gal staining. (A)
EdnrblacZ/+ cultures show melanin-positive cells, many of
which co-express ß-gal (arrow) although some lack clear ß-gal
staining (arrowhead). (B)
EdnrblacZ/EdnrblacZ cultures lack
melanin-positive cells, although ß-gal-positive cells are present
(arrow).
|
