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Fig. 4. KITL rescues melanoblast differentiation in
EdnrblacZ/EdnrblacZ neural crest
cells. Primary cultures from embryos of the indicated genotypes were
established and kept in the presence of KITL for 2 weeks before fixation. The
cultures were then double-labeled for ß-gal and tyrosinase as described
in Fig. 2. Note the presence of
Tyr/ß-gal double-positive cells in KITL-treated cultures, regardless of
whether they were derived from EdnrblacZ/+ (A) or
EdnrblacZ/EdnrblacZ embryos (B). Scale
bar: 25 µm.
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