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Fig. 1. Ubiquitous overexpression of Wg or activated Arm rescues the polarity of
DME cells in wingless mutant embryos. Confocal images of stage 13
embryos showing the subcellular localisation of Fmi and Dlg (A-D, green and
red, respectively), ßTubulin and Fas3 (A'-D', green and red,
respectively), and Myosin (A''-D''; green, C'',D'', red is
Fas3). (A-A'') In wild-type embryos, the DME cells (outlined by Fas3 in
A') are elongated in the DV direction. Fmi (A) localises at the
membrane, but is excluded from the LE (arrow), and accumulates at the level of
the ANCs (arrowhead). The microtubules bundle and orient in the DV direction
(green in A') and Myosin shows a `beads on a string' pattern at the LE
(A''). (B-B'') In wg– embryos, the cells
are stretched in the AP direction and Fmi, which weakly labels the membrane,
fails to accumulate at the ANCs (B, green). The microtubule bundles are
stretched in the main direction of the cells (B') and the weak Myosin
staining is characterised by the lack of accumulation at the LE (B''). In
embryos ubiquitously overexpressing Wg (C-C'') or Armact
(D-D''), although few cells show a triangular shape, the elongation of
the DME and the more ventrally located epidermal cells (C,D) is rescued. The
localisation of Fmi at the membrane and its concentration in ANCs (green C,D),
the orientation of the microtubule bundles (green C',D') and the
`beads on a string' pattern of Myosin (green, C'',D'', red is Fas3)
are comparable with wild type. In all the figures, anterior is leftwards and
dorsal upwards. as, amnioserosa, ep, epidermis. (A-A'',B',B'')
Modified, with permission, from Kaltschmidt et al.
(Kaltschmidt et al., 2002).
Scale bar: 20 µm.
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