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Fig. 3. Activation of the `canonical' Wg pathway is required in the epidermal cells
but not in the amnioserosa. Subcellular localisation of Fmi, Dlg, Myosin, Fas3
and Wg in wg– embryos overexpressing either Wg or
Armact in the amnioserosa. (A,E) Stage 13 embryos stained for Fmi
(green) and Dlg (red). Although the DME cells are oriented in the DV direction
in the wg>AS>Wg embryos (A), they do not elongate and even
sometimes show a triangular shape (arrowhead). The ventrally located
epithelial cells present an isotropic shape (asterisk). In these embryos,
however, Fmi localises at the membrane and concentrates at the level of the
ANCs as in wild type (compare with Fig.
1A). By contrast, in wg>AS>Armact
embryos (E), DME cells are not oriented in the DV direction but rather
stretched in the AP direction as in wg– embryos. In
these embryos, Fmi shows a `dotty' staining similar to that observed in
wg– embryos (compare with
Fig. 1B). (B,C,F,G) Subcellular
localisation of Myosin in stage 13 (B,F) and stage 15 (C,G) embryos. At a
stage when Myosin already shows accumulation at the LE in wild type (compare
with Fig. 1C), no accumulation
is observed in wg>AS>Wg embryos (B). However, when the
zippering process has been initiated at both ends, accumulation is observed at
the LE (C). By contrast, neither at stage 13 (F) nor at stage 15 (G) does
Myosin accumulate at the LE of wg>AS>Armact embryos.
(D) Subcellular localisation of Wg (green) in a stage 13
wg>AS>Wg embryo. After overexpression, a strong Wg expression
is observed in the amnioserosa and Wg-containing dots are seen on the apical
side of the epithelium up to six cells away from the LE (the cells are outline
in red by Dlg). All panels are single confocal sections, dorsal is upwards and
anterior leftwards. Scale bar: 20 µm.
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