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Fig. 6. Contribution of the Dsh domains to the rescue of dorsal closure features in
wg– embryos. (A-D') Dorsal view of the
cuticles shown in Fig. 5.
Confocal high magnification pictures of late stage 13 embryos
(A'',B'',D'') and early stage 14 (C''), oriented dorsal
upwards and anterior leftwards. (A-A') Cuticle of a
wg– embryo showing an anterior and a anterodorsal
hole (arrowheads), as well as a short and folded cuticle on the dorsal side.
After ubiquitous overexpression of Dsh (B) or Dsh
DEP (C) in
wg– embryos, the length of the dorsal side of the
cuticle is rescued and the anterodorsal hole suppressed. The dorsal cuticle of
wg>da>Dsh embryos (B') shows some warts in addition to a
puckering on the midline, while the dorsal cuticle of
wg>da>DshDEP embryos (C') is disorganised with
occasional warts. By contrast, the dorsal cuticle of
wg>da>DshDIX embryos is very short and a strong dorsal
hole is observed (D-D', arrowheads). The cell organisation, as revealed
by Fmi (green) and Dlg (red) staining (A''-D''), is partially
rescued after overexpression of Dsh (B'') or DshDEP (C''),
with DME cells showing a mild elongation in the DV direction and accumulation
of Fmi at the membrane and weakly at ANCS. No rescue of cell shape or Fmi
localisation is observed following overexpression of DshDIX (compare
D'',A'').
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