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Fig. 4. Spatial and temporal expression of AmphiDmbx deduced by
whole-mount in situ hybridisation. Anterior of whole mounts towards the right;
cross-sections (counterstained pink) viewed from the posterior end of animal.
(A) Lateral view of the embryo at the late gastrula stage (9 hours).
AmphiDmbx expression is detected at the anterior mesendoderm. (B,C)
Lateral views of the embryo at the neurula stage (13 hours, 20 hours).
AmphiDmbx expression is seen in dorsal anterior mesendoderm. (D)
Lateral view of the embryo at the mid neurula stage (24 hours). The anterior
mesendoderm expression extends into Hatschek's diverticula. (E) Lateral view
of the embryo at the late neurula stage (36 hours). Expression is detected in
anterior endoderm, including Hatschek's diverticula, and also in the most
rostral tip of the notochord. (F) Cross-section through f in E,
showing expression in the anterior endoderm. (G) Cross-section through
g in E, showing expression in anterior endoderm and notochord, but
not neural tube. (H) Lateral view of 60-hour swimming larva.
AmphiDmbx is expressed in anterior endoderm including part of the
club shaped gland, endostyle and pharyngeal endoderm. Expression is also
observed in a few dorsal cells of the preoral ciliated pit. No expression is
detected in the neural tube at any stage of development examined. a, anterior;
d, dorsal; v, ventral; r, right; l, left; csg, club shaped gland; Hd,
Hatschek's diverticula; n, notochord; nt, neural tube; pcp, preoral ciliated
pit; pe, pharyngeal endoderm; s1, the first somite.
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