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Fig. 5. Spatial and temporal expression of CiDmbx deduced by whole-mount
in situ hybridisation. Anterior towards the right in all specimens except A.
(A) Lateral view of 32-cell embryo; animal pole towards the right. (B) Dorsal
view of a neurula stage embryo. No CiDmbx expression is observed at
these stages. (C) Lateral view of mid tail-bud stage embryo. CiDmbx
expression is detected exclusively in the neural tube. (D) Dorsal view of the
same developmental stage, showing that expression is in one bilateral pair of
cells. (E) Comparison of expression between CiDmbx (blue stain,
arrowhead) and Ci-Pax2/5/8-A (red stain, arrow) at mid tail-bud stage
(lateral view). CiDmbx is expressed in cells immediately posterior to
cells expressing Ci-Pax2/5/8-A, with no gap and no overlap. (F)
Comparison of Ci-Fgf8/17/18 (blue stain) and CiDmbx (red
stain) at mid tail-bud stage (lateral view) showing co-localised expression
(arrowhead). Additional expression of Ci-Fgf8/17/18 is detected in
the trunk-lateral cells and at the tip of the tail after prolonged staining.
(G) Comparison of CiHox3 (blue stain) and CiDmbx (red stain)
at later mid tail-bud stage embryo (lateral view), showing co-localised
expression (arrowhead). Dorsal (H) and lateral (I) views of swimming tadpole
larva. CiDmbx expression is observed in the visceral ganglion, just
behind the sensory vesicle. o, otolith and ocellar pigment spots (expression
negative); pb, polar bodies (expression negative); vg, visceral ganglion
(expressing CiDmbx).
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