|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 2. Enhancer function is dependent on bHLH factors. (A) Two evolutionarily
conserved blocks, M50 and M100, are found in the M250 enhancer. Mouse, rat and
human sequences of M50 and M100 are aligned, and nucleotide coordinates of the
mouse and human sequences are indicated. Two canonical E box sites are marked
with asterisks (*). (B) Mutations were introduced into the E boxes
as shown, and functional activity was tested in the context of the
M250+
NheI:GFP construct shown in
Fig. 1B,F. (C,D) GFP expression
in HH stage 24 chick embryos following electroporation of each reporter
construct. (E,F) GFP expression in E11.5 transgenic mouse embryos containing
wild-type or E box mutant constructs.
|
