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Fig. 6. Repressors interacting with dispersed elements suppress ectopic Hb9 expression. (A) Distal fragments were linked to the proximal –1386 segment ({Delta}NheI) of Hb9 to map elements involved in repressing ectopic gene expression. (B-E) DNA clones were electroporated into chick embryos, and immunocytochemistry was used to detect lacZ (green) and Hb9 (red) at HH stage 24. White bar indicates dorsal limit of Hb9+ motoneuron population. (B) The proximal segment of Hb9 drives widespread reporter expression. (C) The 1 kb distal fragment does not restrict the ectopic expression of Hb9. (D) The 4.6 kb distal fragment limited reporter expression to motoneurons. (E) The 2.5 kb distal fragment expressed nlacZ in motoneurons and a few cells in the V2 interneuron region. (F) The proximal 550 nucleotide segment of Hb9 activates transcription throughout the spinal cord. In non-motoneurons (non-MNs), this activation is suppressed by factors that require elements in the 4.6 kb distal region of the promoter.





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