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Fig. 1. Search for enhancers of Otx2 expression in ectoderm at 5' upstream by BAC transgenesis. (A) BAC #1/lacZ reporter gene. A of the ATG translation start codon is taken as +1 bp. (B) ß-Gal expression in ectoderm at each stage by BAC #1 (a-c) and expression in cephalic mesenchyme (d) by the 1.8 kb promoter. Arrows indicate expression by the 1.8 kb promoter. Scale bars: 100 µm in a,b; 400 µm in c,d.





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