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Fig. 3. Deletion analysis of EP enhancer. (A) Dissection of the #12 genomic fragment for EP and AN enhancers. The number of ß-gal-positive embryos among transient transgenic embryos obtained is indicated on the right (ND, not determined). Blue boxes indicate regions demonstrating in excess of 80% identity over more than 100 bp between mouse and human; yellow boxes indicate the regions demonstrating in excess of 80% identity over more than 100 bp between mouse and Xenopus. (B) ß-Gal expression driven by the EcoRV/BglII 2.3 kb fragment with 1.8 kb promoter (a-d) and with hsp68 promoter (e-h) at E3.5 (a), E5.5 (b), E6.5 (c-f) and E7.75 (g,h). In lateral views (c,e,g), anterior is leftwards; (d,f) cross-sections at the level indicated in b,d, respectively; (h) a sagittal section. Arrows in d indicate ß-gal expression in anterior visceral endoderm by the 1.8 kb mouse Otx2 promoter; this expression is absent with hsp68 promoter (f). The expression is absent in definitive anterior mesendoderm with hsp68 promoter (an arrow in h, compare with Fig. 2B, part d). Scale bars: 100 µm.





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