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Fig. 6. AN enhancer mutant phenotype. (A) Histological features of forebrain defects in Otx2^AN/– (b) and Emx2^–/–Otx2^AN/AN (c) mutants at E12.5; (a) wild-type brain. These phenotypes were examined with both the Otx2^AN mutant in which the neo insert remained and the Otx2^AN mutant in which the insert was deleted by Cre recombination. No differences were found, and the following marker analyses were performed with the mutant that retained the neo insert. The E12.5 Otx2^AN/– phenotype was variable, and a severe phenotype is shown in b. Scale bars: 400 µm. (B) Marker analyses of wild-type (a,c,e,g,i,k) and Otx2^AN/– mutant (b,d,f,h,j,l) embryos at E7.5 (a,b), E8.5 (c-h) and E9.5 (i-l); expression of Six3 (a-d), Fgf8 (e,f), Gbx2 (g-j) and Emx2 (k,l). The E9.5 phenotype was variable; severe examples are shown. Scale bars: 100 µm in a,b; 150 µm in c-h; 400 µm in i-l. (C) Marker analyses of diencephalon in E11.5 wild-type (a,c,e,g,i) and Emx2^–/–Otx2^AN/AN mutant (b,d,f,h,j) embryos; expression of Pax6 (a,b), Dlx1 (c,d), Gbx2 (e,f), Tcf4 (g,h) and Lhx1 (i,j). Scale bars: 400 µm.

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