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Fig. 6. Targeted disruption of FM2 enhancer. (A) Diagrammatic representation of wild-type Otx2 allele, targeting vector and recombinant allele. The black box indicates the BsmI-BamHI 1.0 kb region (FM2) to be replaced with a neomycin-resistant gene (Neo) (see Fig. 3A for others). (B) Otx2{Delta}FM2/{Delta}FM2 (a,b) and Otx2{Delta}FM2/– (c,d) phenotype at E12.5 (a,c) and E15.5 (b,d). The phenotypes were examined with both the Otx2{Delta}FM2 mutant in which the neo insert remained and the Otx2{Delta}FM2 mutant in which the insert was deleted by Cre recombination. No differences were found, and the following, marker analyses were performed with the mutant that retained the neo insert. (C) Marker analysis of Otx2{Delta}FM2/– phenotype at E10.5; Otx2 (a), Pax6 (b), Fgf8 (c) and Emx2 (d) expression (see Fig. 3C for the expression in wild-type embryos). Scale bars: 400 µm.





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