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Fig. 3. Forced expression of FGFR1-Fc and FGFR2-Fc prevents feather placode
development. (A-H) Embryos were injected with the virus into the subectodermal
mesenchyme of the back at HH18 and analyzed for expression of Wnt6,
Shh and Bmp2 at HH29-30 by whole-mount RNA in situ
hybridization. All embryos infected with RCAS-FGFR1-Fc or RCAS-FGFR2-Fc show a
region devoid of any punctate expression (brackets). (I,J) Sections through
the embryo shown in G stained with an antibody against the viral gag protein
demonstrates viral infection in both the nude patch and adjacent bud
containing regions. The asterisks in J indicate infected cells in the
ectoderm. (M,M') HE stained sections through a control (M) and nude
region of an RCAS-FGFR1-Fc infected embryos (M') showing failure to form
ectodermal placodes (ep) and dermal condensates (dc) in infected tissue. ec,
ectoderm that has not formed a placode; dd, thickened dermis. (K,L) Embryos
were injected subectodermally at HH23-24 and analyzed for Bmp2 (K)
and subsequently Fc expression (L, both in blue) by double
whole-mount RNA in situ hybridization. Embryos showed loss of Bmp2
expression and a failure to form buds in the infected area (indicated by white
dotted line). (N,O) Embryos injected at HH26 show normal bud development and
Bmp2 expression in infected areas. Infection is monitored by
subsequent Fc detection in Bmp2/Fc double stained embryos
(indicated by white dotted circles in N and O).
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