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Fig. 4. Inhibition of FGF signaling in vitro prevents feather placode induction but
not the maintenance of established feather buds. Skin explants isolated at
HH28, prior to placode formation (ppf; A-H), were cultured in the presence of
DMSO or Su5402 for 16 hours in vitro and analyzed by whole-mount in situ
hybridization for Shh, Bmp2, ß-catenin or
Fgfr1 expression. Explants isolated at HH29, when the first buds have
been established (eb; I-N), were separated along the midline as indicated by
the doted line in A, and halves were either fixed directly after isolation
(I,K,M) or cultured for 16 hours in the presence of Su5402 (J,N) or DMSO (L),
and expression of Shh (I-L) or Bmp2 (M,N) was compared in
the two halves.
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