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Fig. 4. Ectopic human SPRY2 in the ureteric bud leads to deregulated
expression of Wnt11/Gdnf and Foxd1 and is associated with
reduced branching. (A) Gdnf is expressed in the kidney mesenchyme,
its gene encoding the Ret receptor and another gene Wnt11 in
the ureteric bud (B,C). At the same developmental stage, Gdnf
(A') and Wnt11 (C') expression is reduced in response to
human SPRY2 expression, while Ret remains unchanged
(B'). Note that there are less ureteric tips in the kidneys expressing
human SPRY2, as indicated by the tip markers (compare B,C with
B',C'). While Bmp4 expression shows no notable changes
(D,D'), the stromal marker Foxd1 is clearly reduced due to
human SPRY2 expression (compare E with E'). Mouse
Spry1 expression remains constant irrespective of expression of the
human SPRY2 transgene (F,F'). (A-F') E12.5. Scale bars:
100 µm. WT, wild type; TG, explants containing human SPRY2.
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