spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 2. MiR159 directs the cleavage of MYB33 transcripts and regulates MYB33 levels. (A) Constructs specifying miR159a, MYC-MYB33 and MYC-mMYB33 RNAs and driven by the 35S promoter. 35S:GFP was used as control for co-Agro-inoculation. (B) RNA gel-blot analysis of miR159, MYC-MYB33 and MYC-mMYB33 RNA forms (full length MYC-MYB33a and cleaved 3'-MYB33b transcripts) in N. benthamiana leaves co-Agro-inoculated with different combinations of 35S:miR159a, 35S:MYC-MYB33, 35S:MYC-mMYB33 and 35S:GFP as indicated. (C) Immunoblot analysis of total proteins (20 µg) from N. benthamiana leaves co-Agro-inoculated as in B. Goat anti-myc antiserum and a peroxidase-conjugated anti-goat IgG were used as primary and secondary antibodies, respectively. The lower panel shows a GFP immunoblot using the same extracts (loading control).





Right arrow Return to article