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Fig. 1. (A) Drawing of the dorsal view of an E8.5 Institute of Cancer Research (ICR) mouse embryo with 5 somites. Labelling/graft sites (*) were located on the left side of the embryo at the lateral margin of the neural plate, where the neural tube had not yet completely closed [prorhombomere b (ProRhB), prorhombomere c (ProRhC) and at levels of somites 1-3], or in the dorsal part of the closed neural tube [at levels of somites 4 (S4) and 5 (S5)]. The neural crest of ProRhB was injected or grafted just rostral to the position of the otic placode. The definition of prorhombomeres follows Osumi-Yamashita et al. (Osumi-Yamashita et al., 1996). PreOs, preotic sulcus; PostOs, postotic sulcus. (B) Lateral view of an E8.5 ICR embryo immediately following grafting with a DiI-labelled neural crest fragment (arrow). (C) Dorsal view of an embryo 48 hours following grafting. The graft site is indicated by the arrow. Scale bars: 250 µm in B; 160 µm in C. ot, otic vesicle.





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