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Fig. 2. Lateral views (A-C,E) and transverse sections (D,F) of ICR embryos after 48 hours in vitro. (A) An embryo which was injected with DiI at prorhombomere b (ProRhB) (arrow marks injection site) appears normal at 48 hours. (B) In this embryo, a stream of DiI-labelled cells (white arrows) is observed extending from the labelling site (black arrow) to the second pharyngeal arch (2). (C) Following injection of another embryo in prorhombomere c (ProRhC) (black arrow), DiI-labelled cells (white arrows) are seen extending into the third pharyngeal arch (3). (D) A section through the level of the labelling site in C shows DiI-labelled cells (white arrows) in a dorsolateral pathway that extends from the dorsal side of the neural tube to the level of the developing pharynx (ph). (E) Grafting of a DiI-labelled neural crest fragment into ProRhC (black arrow), produces a narrow stream of labelled cells (white arrows) passing from the graft site to the third pharyngeal arch (3). (F) A section through the graft site of the embryo in E shows a similar distribution of labelled cells (white arrows) as in the DiI-injected embryo (C,D). Scale bars: 160 µm in A-C,E; 80 µm in D,F. da, dorsal aorta; ot, otic vesicle; ph, pharynx.

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