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Fig. 4. Lateral views (A,B,D) and transverse section (C) of ICR embryos after 48 hours in vitro. (A,B) DiI-labelled cells (white arrows) are seen spreading from the labelling site (black arrow) to the third (3), fourth (4) and sixth (6) pharyngeal arches after injection at the level of the first somite (A), or after grafting with a DiI-labelled neural crest fragment at the level of the fourth somite (B). Some labelled cells are found in the aortic sac and cardiac outflow tract (oft) (arrowheads in B). (C) Section through the level of the graft site of the embryo in B confirms that some labelled cells (arrows) have reached the proximal end of the oft ventral to the pharynx (ph). (D) DiI injection of neural crest at the level of the fifth somite (black arrow) produces labelled cells in the foregut (arrowhead) but not in the pharyngeal arches nor any cardiac structures. Scale bars: 160 µm in A,B,D; 80 µm in C. nt, neural tube; ot: otic vesicle.

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