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Fig. 7. Number of WGA-Au-labelled cells in different regions of ICR and splotch mutant embryos at different somite stages following labelling of the neural crest in prorhombomere a (ProRhA) or prorhombomere b (ProRhB) (A-C) or at the level of the second somite (D-F). Note that the scale on the y-axis in D is different from other graphs. ICR embryos and wild type (+/+), heterozygous (Sp^2H/+) and homozygous (Sp^2H/Sp^2H) embryos from splotch litters all show similar numbers of labelled cells in the trigeminal ganglia (A), the facio-acoustic ganglia (B) and the second pharyngeal arches (C) at the 9-, 12-13, 16-17 and 20-21-somite stage, following labelling of neural crest at ProRhA or ProRhB. In contrast, labelling of the neural crest at the level of the second somite produced significantly fewer labelled cells in splotch mutants in the following regions: medial pathway (i.e. sclerotomal mesenchyme between the somite and the neural tube) of the homozygous (Sp^2H/Sp^2H) embryos with 9, 12-13, 16-17 and 20-21 somites (D); peri-aortic mesenchyme of heterozygous (Sp^2H/+) and homozygous (Sp^2H/Sp^2H) embryos with 12-13, 16-17 and 20-21 somites (E); and the mesenchymal region lateral to the developing pharynx of homozygous (Sp^2H/Sp^2H) embryos with 12-13 somites and heterozygous (Sp^2H/+) and homozygous (Sp^2H/Sp^2H) embryos with 16-17 and 20-21 somites (F). The number of labelled cells at each time point was computed from at least 5 embryos (bars: s.e.m.). ^*Significantly different from the value for ICR embryos with the same somite number by Student's two-tailed t-test (P<0.05).

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