QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 8. Transverse sections through wild type (+/+) (A,D,G,J), heterozygous (Sp^2H/+) (B,E,H,K) and homozygous (Sp^2H/Sp^2H) (C,F,I,L) splotch embryos with 7-8 somites (A-C), 12-13 somites (D-F), 16-17 somites (G-I) and 20-21 somites (J-L) after labelling with WGA-Au at the level of the second somite. At 7-8 somites, labelled neural crest cells (arrows in A,B) are seen emigrating from the neural tube into the dorsal mesenchyme underneath the surface epithelium in both +/+ and Sp^2H/+ embryos. In contrast, Sp^2H/Sp^2H embryos do not show any labelled cells in the same mesenchymal region (C). At 12 to 13 somites (D,E), more labelled cells can be seen migrating in the dorsolateral and medial pathways (arrows in D,E) in +/+ (D) and Sp^2H/+ embryos (E). Some labelled cells have already reached the peri-aortic region. Sp^2H/Sp^2H embryos at the same stage (F) show fewer labelled cells in the cranial mesenchyme, which are mainly located dorsal to the dorsal aorta (arrow in F). At 16-17 somites, +/+ (G) and Sp^2H/+ (H) embryos contain labelled cells (arrows in G,H) that have passed beyond the dorsal aorta and are localised lateral to the developing pharynx. In contrast, labelled cells in Sp^2H/Sp^2H embryos (arrows in I) are now present in regions lateral to the dorsal aorta. By 20 to 21 somites, labelled cells have traversed to the ventral side of the pharynx in both +/+ (J) and Sp^2H/+ (K) embryos whereas, in Sp^2H/Sp^2H (L) embryos, labelled cells are located dorsolateral to the pharynx, but not at more ventral locations. Scale bar: 50 µm. da, dorsal aorta; nt, neural tube; ph, pharynx.

Return to article