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Fig. 9. Transverse sections through recipient embryos 24 hours after receiving an orthotopic WGA-Au-labelled neural crest graft at the level of the second somite. Graft combinations (donorrecipient) are: (A,B) +/++/+; (C,D) Sp^2H/Sp^2H+/+; (E,F) Sp^2H/Sp^2H Sp^2H/+; (G) Sp^2H/Sp^2HSp^2H/Sp^2H. Note that all grafts (outlined by arrowheads in A,C,E,G) successfully incorporated into the dorsal neural tube of recipient embryos. In the +/++/+ (B) and Sp^2H/Sp^2H+/+ (D) embryos, labelled graft-derived cells (arrows) can be seen migrating lateral to the dorsal aorta (da), reaching as far ventrally as the pharynx (ph). In the Sp^2H/Sp^2HSp^2H/+ embryo (F), labelled cells (arrows) are migrating, but have not progressed beyond the dorsal aorta. Most strikingly, in the Sp^2H/Sp^2HSp^2H/Sp^2H embryo (G), only one labelled cell (arrow in G) has emerged from the graft, and has migrated only a short distance lateral to the neural tube (nt). Scale bars: 25 µm in A-C,E; 13 µm in D,F,G. da, dorsal aorta; nt, neural tube; ph, pharynx.

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